Fig. 4. Oocyte-inherited H3K27me3 domains are absent in E6.5 ExE.

(A) Average enrichment of H3K27me3 [log₂(maternal/paternal)] at the maternal H3K27me3 domains in morulae, WT ExE, and DNMT3A/3B DKO ExE. Maternal H3K27me3 domains previously identified in BDF1 × PWK IVF morula embryos (n = 4134) (31) were used to generate the plot. Note that the genetic background of the morula sample is the same as the ExE samples. For both WT and DNMT3A/3B DKO, two H3K27me3 CUT&RUN replicates were pooled for the analyses. For the DKO group, embryos #1 and #5 (Fig. 3B) were used for H3K27me3 CUT&RUN experiments separately and pooled for analysis. (B to D) Genome browser views of allelic H3K27me3 enrichment at Gab1, Platr20, Slc38a4, Smoc1, Phf17, and Sfmbt2 loci. The arrows in (C) points to the maternal allele–biased H3K27me3 peaks that are observed in WT but not in the DNMT3A/3B DKO ExE.