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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Breast Cancer Res Treat. 2019 Sep 30;179(1):241–249. doi: 10.1007/s10549-019-05441-3

Figure 1:

Figure 1:

Detection of ERβ using the PPZ0506 antibody via western blotting. A). Western blot analysis of whole cell lysates (40 μg) obtained from indicated cell lines grown in the absence and presence of doxycycline using the ERβ PPZ0506 and Flag antibodies. GAPDH is shown as a protein loading control. B). Western blot analysis of whole cell lysates (40 μg) obtained from U2OS cells that were transiently transfected with either a full-length (FL) ERβ expression vector, or expression vectors for ERβ variants 2, 3, 4 and 5.