Figure 4. The expression of junb is downregulated by TGF-β signaling inhibition.
Tadpoles were treated with a medium containing 12.5 μM SB-505124 or DMSO (Control) from 1 h before tail amputation and cultured until 1, 2 and 6 hpa. (A) WISH analysis of junb in SB-treated tadpoles. N ≥ 29 for each sample. (B) qPCR analysis of junb in SB-treated tadpoles. The expression of junb was normalized against rps18 expression, and then against control samples. Scale bar: 50 μm. **P < 0.01, *** < 0.001, Student’s t-test.