Fig. 4.

FOXO3-dependent apoptosis is suppressed by CBX in low-stage NB cells. a Quantitative RT-PCR analyses of BIM and DEPP expression in SH-EP/FOXO3 cells treated with 20 nM 4OHT in combination with 120 µM CBX for six hours. Shown are means ± s.e.m. of three independent experiments. Statistical analysis was done with the Student’s unpaired t test; **P < 0.025, ***P < 0.01 compared with the corresponding control. b Immunoblot analyses of BIM, NOXA, and DEPP expression in SH-EP/FOXO3 cells treated with 50 nM 4OHT and with indicated concentrations of CBX for eight hours. GAPDH served as loading control. Densitometric analyses of BIM, NOXA, and DEPP expression relative to GAPDH were done with the ImageJ 1.48 software. Untreated cells were set as 100%. c SH-EP/FOXO3 cells were treated with 20 nM 4OHT and with indicated concentrations of CBX for 48 hours. PI-FACS analyses were performed to detect apoptotic cells. Shown are mean values ± s.e.m. of three independent experiments. Statistical analysis was done with the Student’s unpaired t test; ***P < 0.01 compared with the 4OHT-treated control