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. 2019 Oct 4;39(5):975–986. doi: 10.1038/s41388-019-1042-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — NRARP promotes Wnt signaling in T-ALL cells. a Analysis of WNT signaling activity by measuring β-catenin and pβ-catenin Ser675 protein levels by WB in T-ALL cell lines with and without NRARP overexpression. b Effects of Wnt inhibition in the proliferation of T-ALL cells overexpressing or not NRARP using the inhibitor PRI-724. One of three independent assays performed in triplicate is shown. c Analysis of LEF1 protein levels in T-ALL cell lines upon NRARP overexpression. LEF1 has several isoforms due to an alternative promoter and alternative splicing. d Effects of LEF1 knockdown in β-catenin, pβ-catenin Ser675, and cMYC protein levels in T-ALL cell lines with and without NRARP overexpression. e Effects of LEF1 knockdown in the proliferation of T-ALL cells overexpressing or not NRARP. One of three independent assays performed in triplicate is shown. Quantitative analysis of f NRARP:LEF1 interactions and g LEF1-β-catenin interactions performed by the quantification of cells with and without PLA signals per cell per microscope field. The combination of three independent assays and the analysis of five microscope fields per assay are shown. In b, e, f, and g data represent the mean ± SEM. Statistical values were obtained using the Student’s t test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001