Fig. 5.

NRARP negative impact on primary T-ALL cells associates with Notch pathway activation status. Analysis of primary and patient-derived xenograft (PDX) T-ALL cell proliferation a and b viability upon delivery of NRARP recombinant protein (rNRARP). Samples with NOTCH1 mutations/high levels of NICD1 are depicted in orange and samples NOTCH1-WT/low levels of NICD1 in blue. c mRNA analysis of Notch1 and Wnt signaling transcriptional targets by quantitative-PCR, in primary and PDX T-ALL cells treated with rNRARP. In a and b data represent the mean ± SEM. Statistical values were obtained using the Student’s t test. *p < 0.05, **p < 0.01