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. 2019 Oct 9;39(5):1112–1124. doi: 10.1038/s41388-019-1046-5

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© The Author(s) 2019, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — HUNK regulates EGFR-directed metastatic phenotypes in BT20 cells. a Western blot showing the expression levels of Snail (HUNK shRNA1 p < 0.05; HUNK shRNA2 p < 0.05), and E-Cadherin (HUNK shRNA1 p < 0.05; HUNK shRNA2 p < 0.05) in control, HUNK shRNA1, and HUNK shRNA2 BT20 cells. b Mammosphere formation assay of 500 control, HUNK shRNA1, and HUNK shRNA2 BT20 cells at original/day 7 (HUNK shRNA1 p < 0.01; HUNK shRNA2 p < 0.001) and renewal/day 14 (HUNK shRNA1 p < 0.05; HUNK shRNA2 p < 0.001). c Transwell migration (HUNK shRNA1 p < 0.05; HUNK shRNA2 p < 0.01) and (d) invasion (HUNK shRNA1 p < 0.05; HUNK shRNA2 p < 0.05) assays showing cell migration and invasion of 250,000 control, HUNK shRNA1, and HUNK shRNA2 BT20 cells after 24 h. All graphs show mean ± SEM (n = 3 for a–c, n = 4 for d)