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. 2020 Jan 23;11:9. doi: 10.3389/fimmu.2020.00009

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Copyright © 2020 Cohen, Lindsay, Xu, Lin and Wasserman.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunofluorescence staining of F. oxysporum hyphae. Images showing various staining patterns of hyphae incubated with FLAG-Dso2 (A–D) or w¹¹¹⁸ hemolymph (E–H) and then stained with mouse α-FLAG M2 (1:200) and donkey α-mouse Alexa 555 (1:400). DAPI marks fungal DNA. Scale bar is 10 μm. Images were generated as focused images from Z-stacks.