Figure 6.

Triple-knockdown of PGRPs leads to a decrease in mosquito survival and increases fungal proliferation. Survival curves of triple-silenced mosquitoes (targeting PGRP-LA, PGRP-LD, and PGRP-S1) under the context of infection with either (A) B. bassiana or (B) I. javanica. Estimation of fungal loads in PGRP triple-silenced mosquitoes infected with (C) B. bassiana or (D) I. javanica at five days post-infection. Survival curves represents at least four independent experiments and data was analyzed with Log-rank Test (GraphPad Prism 8). Fungal loads were analyzed from two independent experiments. The statistical significance of fold change values was determined on log₂-transformed values via Mann-Whitney test. Error bars indicate the SEM. *P < 0.05.