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. 2020 Jan 29;10:1454. doi: 10.1038/s41598-020-58206-0

Figure 2.

Figure 2

Effect of ABA on the p-AMPK/AMPK ratio and on AMPK, Akt and PGC1α expression levels in L6 cells and in murine muscle. (a) Serum-starved L6 myoblasts were incubated without (white bars) or with 100 nM ABA (black bars) for 60 min. pAMPK (Thr172) and pAkt (Ser473) and the respective total proteins were analyzed by Western blot. The mean ± SD from at least 3 experiments is shown. *p < 0.03 relative to control. (b,c) L6 cells stably infected with a scramble (shRNA-SCR) or with a LANCL2-targeting shRNA (shRNA-L2) were incubated without (control) or with 100 nM ABA for 60 min. (b) Western blot analysis of the pAMPK (Thr172)/AMPK ratio. (c) RT-PCR analysis of PGC-1α mRNA. The mean ± SD from 3 experiments is shown. *p < 0.01 relative to control, #p < 0.02 relative to control. (d,e) Small samples of femoral quadriceps were incubated without (control) or with 100 nM ABA for 30 or 60 min; the pAMPK/AMPK ratio was analyzed by Western blot (d) and the mRNA levels of AMPK and of PGC-1α were determined by RT-PCR (e). The mean ± SD from 3 experiments is shown. *p < 0.01 relative to control; **p < 0.01 and #p < 0.03 relative to the respective time zero.