Table 2.
Raw basal values of mean arterial pressure (MAP), heart rate (HR) and tail temperature (TT) before and after the microinjection of CoCl2 into the DMH in rats subjected to RS.
| Baseline values before RS | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CoCl2 (mM/100nl) | n | MAP | HR | TT | |||||||||
| Before ± SEM (mmHg) | After ± SEM (mmHg) | t= | P= | Before ± SEM (bpm) | After ± SEM (bpm) | t= | P= | Before ± SEM (°C) | After ± SEM(°C) | t= | P= | ||
| 0 | 5 | 103.0 ± 2.9 | 101.6 ± 2.7 | 1.9 | 0.1 | 373.3 ± 14.2 | 384.3 ± 6.7 | 1.2 | 0.2 | 30.6 ± 0.7 | 29.6 ± 0.7 | 1.5 | 0.1 |
| 1 | 5 | 93.1 ± 4.9 | 100.9 ± 5.4 | 3.3 | 0.02# | 349.3 ± 8.0 | 415.0 ± 21.1 | 3.7 | 0.01# | 29.9 ± 1.1 | 30.2 ± 1.6 | 0.4 | 0.6 |
| Autonomic response during RS | |||||||||||||
| CoCl2 (mM/100nl) | n | MAP | HR | TT | |||||||||
| RS average ± SEM (mmHg) | t= | P< | RS average ± SEM (bpm) | t= | P< | RS average ± SEM (°C) | t= | P< | |||||
| 0 | 5 | 110.5 ± 0.5 |
16.9 |
0.0001* |
442.7 ± 2.6 |
6.7 |
0.0001* |
27.5 ± 0.2 |
7.5 |
0.0001* |
|||
| 1 | 5 | 100.3 ± 0.3 | 421.7 ± 1.6 | 30.1 ± 0.1 | |||||||||
Values are raw mean and ± SEM. #significant difference before and after treatment, Student’s paired t test, P < 0.05. No significant difference was found in the MAP basal values of CoCl2-treated animals when compared to the ACSF one (0 versus 1) before (t = 1.7, P = 0.1) and after (t = 0.10, P = 0.9) pretreatment into DMH. No significant difference was found in the HR basal values of CoCl2-treated animals when compared to the ACSF one (0 versus 1) before (t = 1.4, P = 0.1) and after (t = 1.4, P = 0.2) pretreatment into DMH. No significant difference was found in the TT basal values of CoCl2- treated animals when compared to the ACSF one (0 versus 1) before (t = 0.5, P = 0.6) and after (t = 0.3, P = 0.7) pretreatment into DMH. Student’s unpaired t test), P > 0.05. *significant difference when compared ACSF-RS and CoCl2-RS groups, Student’s unpaired t test, P < 0.05.