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. 2020 Jan 29;11(1):73. doi: 10.1038/s41419-020-2256-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A Cortex expression of 4HNE determined by immunohistochemical staining after 48 h of indicated treatment, and the statistical analysis of the positive expression rate of each group (a–c). Scale bar = 50 μm. B The expression of VDR on renal tissue (d–f) were determined by immunohistochemical staining after 48 h of cisplatin with paricalcitol pretreatment, followed by the semi-quantitative statistical analysis of each group. Scale bar = 50 μm. C Representative images of mitochondrial injury at 48 h under indicated treatment were observed by TEM. Scale bar = 5 μm in g–i. Scale bar = 1 μm in j–l. D Quantitative analysis of the expression levels of MDA in kidney tissues of the three indicated groups. E Western blots analysis of GPX4 and VDR in renal tissue lysates from these three groups. The ratio of the optical density of GPX4 and VDR to β-actin were statistically analyzed. The data are presented as the mean ± SD, *P < 0.05, **P < 0.01, n = 8.