TABLE 1.
Mutational analysis and fermentation profiles of anaerobically adapted ΔptsG mutants.
| Metabolites (mM) | ||||||||||
| Strain | Genotype | Fermentation time (h)a | OD600nm | Residual D-glucoseb | Acetate | Ethanol | Formate | Lactate | Succinate | Succinate ratioc (fold) |
| BW25113 | Wild-type | 6 | 5.63 ± 0.13 | NDd | 38.6 ± 0.2 | 36.4 ± 0.3 | 73.1 ± 0.1 | 10.3 ± 0.1 | 6.0 ± 0.0 | 0.04 (1.0) |
| HK620* | ΔptsG | 48 | 2.68 ± 0.07 | ND | 40.8 ± 0.2 | 29.1 ± 0.2 | 58.3 ± 0.8 | 5.21 ± 0.4 | 21.8 ± 0.6 | 0.15 (3.9) |
| HK622* | ΔptsG mlc:IS5 (1203 bp) | 9 | 4.98 ± 0.37 | ND | 35.9 ± 0.4 | 34.6 ± 0.4 | 65.0 ± 1.2 | 18.7 ± 0.1 | 7.4 ± 0.1 | 0.05 (1.3) |
| HK623 | ΔptsG Δmlc (∼6 kb deletion) | 9 | 5.01 ± 0.15 | ND | 35.8 ± 0.2 | 33.1 ± 1.8 | 64.6 ± 0.4 | 17.6 ± 0.6 | 7.2 ± 0.1 | 0.05 (1.3) |
| HK633 | ΔptsG mlc:IS1 (768 bp) | 9 | 5.18 ± 0.24 | ND | 35.5 ± 0.3 | 31.8 ± 1.0 | 64.2 ± 0.9 | 17.8 ± 0.9 | 7.2 ± 0.1 | 0.05 (1.3) |
| HK635* | ΔptsG (adapted) mlc C904T (Q302Z) | 9 | 5.06 ± 0.28 | ND | 35.8 ± 0.3 | 34.5 ± 1.1 | 64.7 ± 0.6 | 17.1 ± 0.2 | 6.9 ± 0.1 | 0.05 (1.2) |
| HK638* | ΔptsG (adapted) mlc C649T (Q217Z) | 9 | 4.86 ± 0.09 | ND | 35.7 ± 0.3 | 34.9 ± 0.4 | 63.2 ± 0.5 | 17.7 ± 0.6 | 7.2 ± 0.2 | 0.05 (1.3) |
| HK639 | ΔptsG (adapted) mlc Δ1101G | 9 | 5.14 ± 0.22 | ND | 36.0 ± 0.1 | 35.1 ± 0.1 | 64.0 ± 0.4 | 17.0 ± 0.1 | 7.1 ± 0.0 | 0.05 (1.2) |
| HK641 | ΔptsG (adapted) mlc C43T (G14Z) | 9 | 5.14 ± 0.14 | ND | 35.7 ± 0.1 | 35.3 ± 0.3 | 63.5 ± 0.2 | 17.6 ± 0.7 | 7.2 ± 0.2 | 0.05 (1.2) |
*Whole genomic sequencing was performed. aFermentation time (h) when glucose was completely consumed. bResidual D-glucose concentration. 50 mM glucose was added initially in the fermentation medium. cCalculated as succinic acid/(succinate + lactate + formate + acetate + ethanol). dND, not detected.