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. 2020 Jan 23;9:1534. doi: 10.3389/fonc.2019.01534

Figure 5.

Figure 5

ST8176AA1 induces cell damage and reduces apoptosis resistance in tumor cells. (A) HCS imaging analysis showing that LS174T cells treated with ST8176AA1 exhibit increased expression of damage markers HSP70, phospho-P21 (pP21) and phospho-P53 (pP53), compared to trastuzumab. ST8176AA1, and trastuzumab were used at 5 μg/mL (3 h incubation). (B) HCS imaging analysis showing that SKOV3 cells treated with ST8176AA1 exhibit reduced expression of the apoptosis resistance biomarkers Bcl2 and BclXL and increased pro-apoptotic cleaved-caspase3 and cleaved-PARP proteins, compared to trastuzumab. ST8176AA1 and trastuzumab were used at 40 μg/mL (3 days incubation). In all panels, insets show specific fluorescence signals within the cells. Draq5 dye staining of nucleus (gray). Each image is representative of at least five fields of duplicate wells. Magnification 60X. Data are from one representative experiment out of two.