Figure 3.
Evaluation of safety and activity of LysRODI and CHAPSH3b using a zebrafish model. (A) Acute toxicity of the proteins was tested by exposure of zebrafish embryos (n = 8) to 1 × MIC of LysRODI or CHAPSH3b. Medium E3 was used as a negative control and 1 mg/ml paracetamol, as a positive control. Bars represent mean ± standard deviation (n = 24) of triplicate experiments. The asterisk indicates statistical differences (p < 0.05) vs untreated control by Chi-square test. (B) Efficacy of LysRODI (I) and CHAPSH3b (II) in a zebrafish model infected with ~105 CFU/fish of S. aureus when treating with 0.5 × MIC, 1 × MIC and 3 × MIC. Kaplan–Meier graphs represent the percentage of cumulative survival zebrafish (n = 18) observed at 24, 48 and 72 h. Asterisks indicate significant differences (p < 0.05) vs. the untreated control according to the Kaplan–Meier cumulative survival plot and statistically compared by the Log-Rank test.