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. 2020 Jan 23;10:1585. doi: 10.3389/fphys.2019.01585

FIGURE 3.

FIGURE 3

The developmental and tissue-specific expression patterns of the PxVgR in P. xylostella. (A,B) The developmental expression profiles of PxVgR transcripts and protein were analyzed by qRT-PCR and Western blot, respectively. The mRNA level was normalized to the P. xylostella RIBP transcripts in qRT-PCR analysis; Proteins (25 ug/lane) were separated using SDS-PAGE, then blotted to the PVDF membrane and probed with the polyclonal antibody against PxVgR. Tubulin was used as the internal reference. Egg; 1-3L: 1-3 instar larvae; 4LM/4LF: 4th instar male/female larvae; MP/FP: male/female pupae; MA/FA: male/female adults. (C,D) The tissue-specific expression patterns of PxVgR transcripts and protein were also analyzed by qRT-PCR and Western blot, respectively. He, head; Th, thorax; Mi, midgut + malpighian tubule; Ov, ovary; Ep, epidermis; Fb, fat body. Data represent with three biological replicates and each replication repeat three times. The bars were shown as the mean ± SE. Different letters mean significant differences (P < 0.05).