TABLE 1.

Primers used for this study.

Primer name	Primer sequence 5′-3′
VgR F1	TCTTTTCTGCACACTTTTAGGG
VgR R1	GGGTCTCGTTGTACTCGTCG
VgR F2	CTACTGCTGCTCTGTCTAGCG
VgR R2	GGGCTGGTCTCGTGGATAAG
VgR F3	CAGGGTTCTACTGACGCTGG
VgR R3	AGGGCAGTCATCTATCCCGT
VgR F4	TACTACATGGGCTACACCTGC
VgR R4	GAGCAGAGGTACTCGCAGTC
VgR F5	CGACCATCAATCCACCCCAA
VgR R5	CCCAGTCTACTGCCACCTTG
3′Race	ACAGACATAGACGAGTGCCG
RIBP F	CAATCAGGCCAATTTACCGC
RIBP R	CTGCGTTTACGCCAGTTACG
qRT-PCR F	ATTGTGACCCCGATGGACTG
qRT-PCR R	TGCAGCGGGTCTCATTCATAG
sgRNA-F1∗	TAATACGACTCACTATAGGAGGCTCCGTGGCGGCGTGCG GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCC
sgRNA-sgR	AAAAGCACCGACTCGGTGCCACTTTTTCAAGTTGATAACGGA CTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAAAAC
VgR sgF1	TGGACTGGGTTCTGACCCTAT
VgR sgR1	TTGTAACCCTCCTTGCCAGA

^∗T7 promoter sequence is underlined, and sgRNA skeleton sequence is in bold.