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. 2020 Jan 23;10:1528. doi: 10.3389/fphar.2019.01528

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Copyright © 2020 Chen, Liu, Li, Han, Tang, Deng, Lai and Wan

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of different concentrations of dihydrotestosterone (DHT) on hair shaft growth of human HFs in vitro. Isolated anagen human HFs were cultured with DHT at various concentrations or vehicle for 10 days. (A) Mean growth rates of hair shafts. Hair shaft length was measured every 2 days (n = 18). (B) Changes in hair bulb morphology during the 10-day experimental period and immunofluorescence staining of Ki67 in HFs cultured with various concentrations of DHT (0, 10^-6 and 10^-7 mol/L) at day 10. (C) Percentages of Ki-67-positive cells in the matrix area (n = 6). The data are presented as the mean ± SD. Each bar represents the mean of three independent experiments performed in triplicate. Compared with the vehicle-treated control group, ^*P < 0.05.