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. 2020 Jan 23;10:1528. doi: 10.3389/fphar.2019.01528

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Copyright © 2020 Chen, Liu, Li, Han, Tang, Deng, Lai and Wan

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of different concentrations of dihydrotestosterone (DHT) on the expression of β-catenin, GSK3β, and p-GSK3β (ser9) in human HFs. The hair follicles (HFs) were fixed and labeled with anti-β-catenin, anti-GSK3β, and anti-p-GSK3β (ser9) primary antibodies, followed by incubation with fluorescent secondary antibodies. (A) The HFs were visualized: green, β-catenin and p-GSK3β (ser9); red, GSK3β (magnification: 200× and 400×). (B, C) The mean fluorescence intensity of β-catenin, GSK3β, and p-GSK3β (ser9) in the matrix area. The data are presented as the mean ± SD (n = 6). Each bar represents the mean of three independent experiments performed in triplicate. Compared with the control group, ^*P < 0.05. Scale bar 50 μm (200×).