Table 2.
Thermodynamic constants of Impα/NLS complexes interactions. Data obtained by ITC assays.
| Complex | Stoichiometry | Kd (μM) | ΔH (kcal/mol) | ΔS (cal/mol/deg) |
|---|---|---|---|---|
| MmImpα/PAC3 | 1.06 ± 0.01 | 0.44 ± 0.05 | 6.63 ± 0.66 | 6.47 |
| MmImpα/PAC3Δminor | No binding | — | — | — |
| MmImpα/PAC3Δmajor | No binding | — | — | — |
| NcImpα/PAC352 | 1.02 ± 0.01 | 0.39 ± 0.07 | −12.17 ± 0.17 | −12.20 |
| MmImpα/NIT2 | 0.95 ± 0.01 | 0.56 ± 0.23 | −0.44 ± 0.19 | −0.02 |
| 0.95 ± 0.01 | 5.74 ± 0.99 | −8.09 ± 0.29 | −0.40 | |
| NcImpα/NIT251 | 1.00 ± 0.01 | 0.56 ± 0.32 | −6.64 ± 0.27 | 0.24 |
| 1.00 ± 0.01 | 9.90 ± 1.10 | −7.04 ± 0.20 | 4.58 |