Table 3.

Binding of nuclear localization sequences to specific binding clusters in Mus musculus importin-α.

Protein		Minor NLS binding site						Linker		Major NLS binding site							Linker	PDB ID
		P0′	P1′	P2′	P3′	P4′				P0	P1	P2	P3	P4	P5		(P3′-P1)
Bipartite NLS
Npl	A	V	K	R	P	A		ATKKAG		Q	A	K	K	K	K	LD	10	1EJY,3UL1
Rb			K	R	S	A		EGSNPPK		P	L	K	K	L	R		11	1PJM
N1N2	R	K	K	R	K			TEEESPLKD		K	A	K	K	S	K		12	1PJN
mCBP80		M	S	R	R			RHSYENDGGQ		P	H	K	R	R	K	TS	13	3UKZ
yCBP80	N	R	K	R	R	G	D	FDEDENYRDFRPR		M	P	K	R	Q	R	IP	18	3UKY
yPRP20			K	R	T	V		ATNGDASGAHRA			K	K	M	S	K		15	4OIH
MAL		L	K	R	K...				...L	K	L	K	R	A	R	LA	34	3TPM
FEN1	S	A	K	R	K	E		PEPKGS		T	K	K	K	A	K	T	10	3UVU
Bimax1	P	R	K	R	P	L	EW	DEDEEP		P	R	K	R	K	R	LW	12	3UKW
Bimax2	RR	R	K	R	K	R	EW	DDDDDP		P	K	K	R	R	R	LD	12	3UKX
53BP1	S	G	K	R	K			LITSEEERSPA				K	R	G	K	S	11	6IU7
PB2	K	R	K	R	D	S		SILTDSQ	T	A	T	K	R	I	R	MA	12	2JDQ, 4UAE
PAC-3	FDA	R	K	R	Q	F		DDLNDFFG		S	V	K	R	R	Q	I	12	6P6E
Monopartite NLS
TPX2			K	R	K	H...					...V	K	M	I	K	L	42	3KND
SV40 TAg	P	K	K	K	R	K	V		P	P	K	K	K	R	K	V		1EJL
SV40-TAg CN	K	K	K	R	K	V			P	P	K	K	K	R	K	V		1Q1S,1Q1T
AR									G	A	R	K	L	K	K	LG		3BTR
PLSCR1											G	K	I	S	K	HW		1Y2A
dUTPase	P	S	K	R	A	R	P		AIS	P	S	K	R	A	R	PA		4MZ5,4MZ6
Ku80									GPT	A	K	K	L	K	T	E		3RZ9
Ku70									NEGS	G	S	K	R	P	K	VE		3RZX
BFDV										Y	R	R	R	R	R	Y		4HTV
CLIC4										V	A	K	K	Y	R	N		3OQS
A89										L	G	K	R	K	Y			4BA3
B54		G	K	R	K	R				L	G	K	R	K	R	H		2YNR
PepTM		K	K	R	R	E	A		P	F	K	K	K	R	R	EA		3L3Q
αIBB									DEQ	M	L	K	K	R	N	VS		1IAL
XPG1	S	L	K	R	K	R				S	L	K	R	K	R			5EKF
XPG2		R	K	R	K	T	R			Q	K	K	R	R	K	LR		5EKG
PLSCR4	S	I	I	R	K	W	N											3Q5U
Guα	SRG	Q	K	R	S	F	SKAFGQ				Q	K	R	S	F	S		3ZIN
A28		R	K	R	G	Y	SVAF				R	K	R	G	Y	S		3ZIO
A58		R	K	R	T	W	RDAF				R	K	R	T	W	R		3ZIP
B6	H	R	K	R	K	F	SDAF				R	K	R	K	F	S		3ZIQ
B141	RQ	R	K	R	K	W	SEAF				R	K	R	K	W	S		3ZIR
Nup50	M	A	K	R	V	A	EKELTD...											2C1M
NIT-2		S	K	R	Q	R	R			S	S	K	R	Q	R	S		6P6A