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. 2020 Jan 29;11:578. doi: 10.1038/s41467-019-14176-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a RNAi screening of peroxisomal genes involved in fasting-induced lipolysis-based Oil Red O (ORO) staining in anterior intestine of C. elegans. ORO staining intensities in young adult RNAi-treated worms under feeding and 8-h fasting conditions were quantified and classified according to the relative fold increase compared to the L4440 control group. n = 21 for feeding L4440; n = 15 for fasting L4440; n = 18 for prx-1; n = 15 for prx-2; n = 16 for prx-3; n = 15 for prx-5; n = 14 for prx-6; n = 19 for prx-10; n = 17 for prx-11; n = 13 for prx-12; n = 16 for prx-13; n = 15 for prx-14; n = 18 for prx-19. b, c Representative images and quantification of ORO staining in anterior intestine of C. elegans with RNAi of atgl-1 and prx-5 in young adult worms under feeding and fasting (8 h). n = 15–20 for quantification. n = 21 for feeding L4440; n = 16 for feeding atgl-1; n = 16 for prx-5; n = 15 for fasting L4440; n = 15 for fasting atgl-1; n = 15 for fasting prx-5. d, e Representative images and quantification of ORO staining in anterior intestine from prx-5 RNAi-treated WT worms (N2) and atgl-1 transgenic worms (ATGL-1 Tg, hj67; Is[atgl-1p::atgl-1::GFP]). n = 15 for L4440 in N2 worms; n = 17 for L4440 in ATGL-1 Tg; n = 19 for prx-5 in ATGL-1 Tg. f Heatmap analysis of Pearson’s coefficients (r) between lipolytic genes (ATGL and HSL) and PEX genes in human adipose tissue based on data from GTEx. Vis visceral; SubQ subcutaneous. g Plots of correlation between PEX5 and all detectable genes in human visceral adipose tissue based on data from GTEx. ACOX1 (green), positive control for correlation of PEX5. h Correlations between expression of PEX5 and ATGL in human visceral adipose tissue based on data from GTEx. All scale bars, 50 μm. Data represent the mean ± SD; ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 in two-way ANOVA followed by Turkey’s post-hoc test c and unpaired two-tailed Student’s t-test e.