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. 2020 Jan 29;10:1443. doi: 10.1038/s41598-020-58084-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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DSB repair factor NBS1-GFP is recruited to HZE ion tracks. Live cell imaging of GFP tagged NBS1 in U2OS cells revealed fast foci formation along the trajectories of ions traversing the nucleus after irradiation with 1 GeV/u Fe ions. The slightly delayed development of some clearly off-track foci (arrowheads) could be observed. Selected time-frames are shown. Image montage was done using ImageJ 1.48 v (https://imagej.nih.gov) and Powerpoint 2010 (Microsoft Corporation, USA)- for full movie see Supplement Movie 1.