TABLE 1.
Strains used in this study
| Species and strain | Strain no. | Description | Reference or source |
|---|---|---|---|
| Pseudomonas aeruginosa | |||
| UCBPP-PA14 | Clinical isolate | 54 | |
| PA14 Δphz | LD24 | PA14 with deletions in phzA1-G1 and phzA2-G2 operons | 60 |
| PA14 Δcco1 cco2 (∆cco) | LD1933 | PA14 with both cco operons cco1 (cbb3-1) and cco2 (cbb3-2) (PA14_44340-PA14_44400) deleted simultaneously | 7 |
| PA14 ∆cio | LD2076 | PA14 with the cioAB (PA14_13030-PA14_13040) operon deleted | This study |
| PA14 PaCco | LD2587 | PA14 with deletions in PA14_01290-01320 (cox/aa3 operon), PA14_47150-47210 (cyo/bo3 operon), and PA14_13030-13040 (cio operon) | 7 |
| PA14 PaCco Δphz | LD2588 | PA14 with only the Cco terminal oxidases remaining in the phenazine-null background; deletions in the phzA1-G1, phzA2-G2, cox/aa3, cyo/bo3, and cio operons | This study |
| PA14 PaCio | LD1989 | PA14 with only the Cio terminal oxidase remaining; deletions in the cox/aa3, cyo/bo3, cco1, and cco2 operons | This study |
| PA14 PaCio Δphz | LD1990 | PA14 with only the Cio terminal oxidase remaining in the phenazine-null background; deletions in the phzA1-G1, phzA2-G2, cox/aa3, cyo/bo3, cco1, and cco2 operons | This study |
| PA14 ΔphzH ΔphzM ΔphzS | LD3122 | PA14 with deletions in the phenazine biosynthetic genes phzH (PA14_00640), phzM (PA14_09490), phzS (PA14_09400), phzA1-G1, and phzA2-G2; also referred to as ΔHMS Δphz | This study |
| ΔphzA1-G1 | |||
| ΔphzA2-G2 | |||
| PA14 ΔphzH ΔphzM ΔphzS | LD3140 | PA14 with deletions in the phenazine biosynthetic genes phzH, phzM, phzS, phzA1-G1, and phzA2-G2 and the terminal oxidase operons of cco1 and cco2; made by mating LD1933 to LD3122; also referred to as ΔHMS Δphz Δcco | This study |
| ΔphzA1-G1 | |||
| ΔphzA2-G2 Δcco | |||
| PA14 attB::MCS-gfp | LD2820 | PA14 with MCS-gfp inserted at the attB site using pLD2722 | 29 |
| PA14 Δphz attB::MCS-gfp | LD2821 | PA14 Δphz with MCS-gfp inserted at the attB site using pLD2722 | 29 |
| PA14 attB::Pcox-gfp | LD2962 | PA14 with promoter of the cox/aa3 operon driving gfp expression inserted at the attB site using pLD2958 | This study |
| PA14 attB::Pcio-gfp | LD2969 | PA14 with promoter of the cio operon driving gfp expression inserted at the attB site using pLD2960 | This study |
| PA14 attB::Pcco-1-gfp | LD2784 | PA14 with promoter of the cco1 operon driving gfp expression inserted at the attB site using pLD2777 | 7 |
| PA14 attB::Pcco-2-gfp | LD2786 | PA14 with promoter of the cco2 operon driving gfp expression inserted at the attB site using pLD2778 | 7 |
| PA14 Δphz attB::Pcox-gfp | LD2963 | PA14 Δphz with promoter of the cox/aa3 operon driving gfp expression inserted at the attB site using pLD2958 | This study |
| PA14 Δphz attB::Pcio-gfp | LD3317 | PA14 Δphz with promoter of the cio operon driving gfp expression inserted at the attB site using pLD2960 | This study |
| PA14 Δphz attB::Pcco-1-gfp | LD2785 | PA14 Δphz with promoter of the cco1 operon driving gfp expression inserted at the attB site using pLD2777 | This study |
| PA14 Δphz attB::Pcco-2-gfp | LD2787 | PA14 Δphz with promoter of the cco2 operon driving gfp expression inserted at the attB site using pLD2778 | This study |
| E. coli | |||
| UQ950 | LD44 | DH5 λpir strain for cloning; F− Δ(argF-lac)169 ϕ80 dlacZ58(ΔM15) glnV44(AS) rfbD1 gyrA96(NaIr) recA1 endA1 spoT thi-1 hsdR17 deoR λpir+ | D. Lies |
| BW29427 | LD661 | Donor strain for conjugation; thrB1004 pro thi rpsL hsdS lacZΔM15RP4-1360 Δ(araBAD)567ΔdapA1314::[erm pir(wt)] | B. Wanner |
| S17-1 | LD2901 | Strr Tpr; F− RP4-2-Tc::Mu aphA::Tn7 recA λpir lysogen | 61 |
| S. cerevisiae InvSc1 | LD676 | MATa/MATα leu2/leu2 trp1-289/trp1-289 ura3-52/ura3-52 his3-Δ1/his3-Δ1 | Invitrogen |