TABLE 2.
Primers used in this study
| Primer | Sequencea | Plasmid made |
|---|---|---|
| LD725 | ccaggcaaattctgttttatcagaccgcttctgcgttctgatCCCCTCAGAGAAGTCAGTCG | pLD1929 (Δcco1 cco2 in pMQ30) |
| LD1063 | gttgcccaggtgttcctgtGGCGGACCACCTTGTAGTTA | |
| LD949 | ggaattgtgagcggataacaatttcacacaggaaacagctTGTAGTCGAGGGACTTCTTGC | |
| LD1064 | taactacaaggtggtccgccACAGGAACACCTGGGCAAC | |
| LD1118 | ccaggcaaattctgttttatcagaccgcttctgcgttctgatTCTTCAGGTTCTCGCGGTAG | pLD1966 (Δcox in pMQ30) |
| LD1119 | aagtgccagtaccaactggcGCAGATCCAGAAGATGGTCA | |
| LD1120 | tgaccatcttctggatctgcGCCAGTTGGTACTGGCACTT | |
| LD1121 | ggaattgtgagcggataacaatttcacacaggaaacagctATCGCGAGACTCATGGTTTT | |
| LD1134 | ccaggcaaattctgttttatcagaccgcttctgcgttctgatCGCTGCTTGTCGATCTGTT | pLD1967 (Δcyo [bo3] in pMQ30) |
| LD1135 | gcgacatgaccctgttcaacCTGACCGGCTACTGGACC | |
| LD1136 | ggtccagtagccggtcagGTTGAACAGGGTCATGTCGC | |
| LD1137 | ggaattgtgagcggataacaatttcacacaggaaacagctCCTCGGCGACCATGAATAC | |
| LD1126 | ccaggcaaattctgttttatcagaccgcttctgcgttctgatTTCAGGTTCTTCGGGTTCTC | pLD2044 (Δcio in pMQ30) |
| LD1187 | aacagcgcgccgaccagcatCTCTTCGTTCGTTTTCAGCC | |
| LD1188 | ggctgaaaacgaacgaagagATGCTGGTCGGCGCGCTGTT | |
| LD1189 | ggaattgtgagcggataacaatttcacacaggaaacagctGCGTTGATGAAGCGGATAAC | |
| LD2247 | gattcgactgcactagtCTCGCCTTGCGGCTGGATGG | pLD2958 |
| LD2248 | gattcgactgcctcgagTCGGGTTCCCCTTATCGTTG | |
| LD2251 | gattcgactgcactagtCGCGGCGCTGGCGGAAACG | pLD2960 |
| LD2252 | gattcgactgcctcgaGGCAACTCCTCTTCAGGTTC | |
| LD2120 | gattcgacatcactagtACGCCCAGCTCCAACAAA | pLD2777 |
| LD2121 | gattcgatgccctcgaGCTAGGGGTTCCACGGTTAAT | |
| LD2122 | gattcgactgcactagtCATCGACTTGCCGCCCAG | pLD2778 |
| LD2123 | gattcgatgccctcgaGCTATGGGCTTCCATCCAC |
a
Underlining indicates introduction of a restriction digest site used for cloning; lowercase letters indicate homologous sequences used for recombination.