TABLE 3.
Plasmids used in this study
| Plasmid | Description | Reference or source |
|---|---|---|
| pMQ30 | 7.5-kb mobilizable vector; oriT sacB Gmr | 57 |
| pLD2722 | Gmr Tetr; flanked by Flp recombinase target (FRT) sites to resolve out resistance cassettes | 7 |
| pFLP2 | Flp recombinase-producing plasmid | 62 |
| pLD1929 | Δcco1 cco2 PCR fragment introduced into pMQ30 by gap repair cloning in yeast strain InvSc1 | 7 |
| pLD1966 | Δcox (aa3) PCR fragment introduced into pMQ30 by gap repair cloning in yeast strain InvSc1 | This study |
| pLD1967 | Δcyo (bo3) PCR fragment introduced into pMQ30 by gap repair cloning in yeast strain InvSc1 | This study |
| pLD2044 | Δcio PCR fragment introduced into pMQ30 by gap repair cloning in yeast strain InvSc1 | This study |
| pLD2958 | PCR-amplified cox (aa3) promoter ligated into pLD2722 using SpeI and XhoI | This study |
| pLD2960 | PCR-amplified cio promoter ligated into pLD2722 using SpeI and XhoI | This study |
| pLD2777 | PCR-amplified cco1 promoter ligated into pLD2722 using SpeI and XhoI | 7 |
| pLD2778 | PCR-amplified cco2 promoter ligated into pLD2722 using SpeI and XhoI | 7 |