FIG 4.

Effect of pH and glycine on growth of the WT and cdaA* strains. The WT and cdaA* strains were grown in THY (A), in THYI medium for transformation (B), in THY with the same HCl concentration as in THYI (pH 7.1) (C), or in THY with the same glycine concentration (0.5%) as in THYI (D). Bacterial growth was monitored by as OD₆₂₀ every 2 h. Data shown are the means from three independent biological replicates. The error bars indicate the standard deviations (SD). *, P < 0.05; **, P < 0.01; ****, P < 0.0001.