TABLE 1.
Strains used in this study
| Strain | Relevant genotype or description | Reference or source |
|---|---|---|
| DH5α | E. coli cloning host | New England BioLabs |
| OI3269 | Bacillus subtilis 168 trpC2 | |
| OI1085 | trpF7 hisH2 metC che+ | 43 |
| PTS083 | ΔcheB | This work |
| PTS084 | ΔcheR | This work |
| PTS097 | ΔcheC | This work |
| PTS135 | ΔcheV | This work |
| PTS324 | ΔmcpA | This work |
| PTS325 | ΔtlpA | This work |
| PTS185 | ΔmcpB | This work |
| PTS186 | ΔtlpB | This work |
| PTS334 | ΔmcpA ΔtlpA | This work |
| PTS187 | ΔtlpB ΔmcpB | This work |
| PTS110 | ΔmcpA ΔtlpA ΔtlpB ΔmcpB | This work |
| OI3545 | Δ10mcp che+ | 24 |
| OI3921 | OI3545 amyE5720::mcpA | 17 |
| OI3605 | OI3545 amyE5720::mcpB | 25 |
| OI3974 | OI3545 amyE5720::mcpC | 17 |
| OI4474 | OI3545 amyE5720::tlpA | This work |
| OI4475 | OI3545 amyE5720::tlpB | This work |
| OI4483 | OI3545 amyE5720::tlpC | This work |
| OI4476 | OI3545 amyE5720::yfmS | This work |
| OI4477 | OI3545 amyE5720::yvaQ | This work |
| OI4482 | OI3545 amyE5720::hemAT | This work |
| OI4479 | OI3545 amyE5720::yoaH | This work |
| PTS163 | OI3545 amyE5720::tlpB284-mcpA | This work |
| PTS165 | OI3545 amyE5720::tlpB362-mcpA | This work |
| PTS368 | OI3545 amyE5720::tlpB261-mcpA | This work |
| PTS493 | OI3545 amyE5720::tlpB238-mcpA | This work |
| PTS505 | OI3545 amyE5720::tlpB180-mcpA | This work |
| PTS500 | OI3545 amyE5720::mcpA180-tlpB284A | This work |
| PTS507 | OI3545 amyE5720::mcpA197-tlpB284A | This work |
| PTS509 | OI3545 amyE5720::mcpA222-tlpB284A | This work |
| PTS441 | OI3545 amyE5720::tlpB[Q273H] [D274E] | This work |
| PTS421 | OI3545 amyE5720::tlpB[K199T] [E200Q] | This work |
| PTS464 | OI3545 amyE5720::tlpB[Q273H] [D274E] [K199T] [E200Q] | This work |
| PTS373 | OI3545 amyE5720::mcpA[H273Q] [E274D] | This work |
| PTS462 | OI3545 amyE5720::mcpA[T199K] [Q200E] | This work |
| PTS481 | OI3545 amyE5720::mcpA[H273Q] [E274D] [T199K] [Q200E] | This work |
| PTS251 | OI3545 amyE5720::tlpB[D274N] | This work |
| PTS276 | OI3545 amyE5720::mcpA[E274Q] | This work |
| GB041 | OI3545 amyE5720::PmcpA::tlpA | Unpublished |