TABLE 2.
Plasmids used in this study
| Plasmid | Description | Reference |
|---|---|---|
| pJOE8999 | Shuttle vector for Cas9 expression and tracrRNA transcription; Kanr | 44 |
| pJSpe | Modified pJOE8999 optimized for Gibson assembly of homology templates; Kanr | This work |
| pPT037 | pJSpe::cheV (cheV knockout vector) | This work |
| pPT039 | pJOE8999::cheB (cheB knockout vector) | This work |
| pPT041 | pJOE8999::cheR (cheR knockout vector) | This work |
| pPT046 | pJSpe::cheC (cheC knockout vector) | This work |
| pPT048 | pJSpe::mcpA tlpA mcpB tlpB (4mcp knockout vector) | This work |
| pPT058 | pJSpe::mcpB (mcpB knockout vector) | This work |
| pPT074 | pJSpe::tlpB (tlpB knockout vector) | This work |
| pPT116 | pJSpe::mcpA (mcpA knockout vector) | This work |
| pPT118 | pJSpe::tlpA (tlpA knockout vector) | This work |
| pPT141 | pJSpe::mcpA-tlpA (mcpA-tlpA knockout vector) | This work |
| pAIN750 | B. subtilis amyE integration vector; Ampr Spcr | 17 |
| pAIN750mcpA | pAIN750::mcpA | 25 |
| pAIN750mcpB | pAIN750::mcpB | 25 |
| pAIN750mcpC | pAIN750::mcpC | 17 |
| pAIN750tlpA | pAIN750::tlpA | This work |
| pAIN750tlpB | pAIN750::tlpB | This work |
| pAIN750tlpC | pAIN750::tlpC | This work |
| pAIN750yfmS | pAIN750::yfmS | This work |
| pAIN750yvaQ | pAIN750::yvaQ | This work |
| pAIN750hemAT | pAIN750::hemAT | This work |
| pAIN750yoaH | pAIN750::yoaH | This work |
| pPT065 | pAIN750::tlpB362-mcpA | This work |
| pPT063 | pAIN750::tlpB284-mcpA | This work |
| pPT143 | pAIN750::tlpB261-mcpA | This work |
| pPT224 | pAIN750::tlpB238-mcpA | This work |
| pPT234 | pAIN750::tlpB180-mcpA | This work |
| pPT233 | pAIN750::mcpA180-tlpB284A | This work |
| pPT236 | pAIN750::mcpA197-tlpB284A | This work |
| pPT237 | pAIN750::mcpA222-tlpB284A | This work |
| pPT129 | pAIN750::tlpB[Q273H] [D274E] | This work |
| pPT162 | pAIN750::tlpB[K199T] [E200Q] | This work |
| pPT202 | pAIN750::tlpB[Q273H] [D274E] [K199T] [E200Q] | This work |
| pPT196 | pAIN750::mcpA[H273Q] [E274D] | This work |
| pPT163 | pAIN750::mcpA[T199K] [Q200E] | This work |
| pPT222 | pAIN750::mcpA[H273Q] [E274D] [T199K] [Q200E] | This work |
| pPT101 | pAIN750::tlpB[D274N] | This work |
| pPT107 | pAIN750::mcpA[E274Q] | This work |
| pGB045 | pAIN750::PmcpA::tlpA | Unpublished |