TABLE 2.
Strains and plasmids used in this study
| Strain or plasmid | Genotype or descriptiona | Reference or source |
|---|---|---|
| Vibrio alginolyticus strains | ||
| VIO5 | Rifr Pof+ Laf− | 55 |
| KK148 | VIO5 flhG (Pof+ Laf− multi-Pof) | 56 |
| NMB191 | VIO5 ΔpomAB (Laf− Mot−) | 57 |
| TH1 | KK148 ΔmotX (multi-Pof Laf− Mot−) | 16 |
| TH2 | KK148 ΔmotY (multi-Pof Laf− Mot−) | 16 |
| TH3 | KK148 ΔmotXY (multi-Pof Laf− Mot−) | 16 |
| TH7 | KK148 ΔflgT (multi-Pof Laf− Fla−) | 21 |
| NMB300 | KK148 ΔpomAB (multi-Pof Laf− Mot−) | 20 |
| NMB337 | KK148 ΔflgO (multi-Pof Laf− Fla−) | 9 |
| NMB352 | KK148 ΔflgBC (51–124) (multi-Pof Laf− Fla−) | This study |
| NMB354 | KK148 ΔflgCC (56–130) (multi-Pof Laf− Fla−) | This study |
| NMB356 | KK148 ΔflgFc (118–242) (multi-Pof Laf− Fla−) | This study |
| NMB357 | KK148 ΔflgGN (8–140) (multi-Pof Laf− Fla−) | This study |
| Escherichia coli strains | ||
| DH5α | F− ϕ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rK− mK+) phoA supE44 λ− thi-1 gyrA96 relA1 | 58 |
| S17-1 | Tpr Smr F− recA thi pro hsdR RP4-2(Tc::Mu-1 Km::Tn7) λpir | 59 |
| β3914 | Kmr Emr Tcr β2163 gyrA462 zei-298::Tn10 | 36 |
| Plasmids | ||
| pBAD33 | Cmr PBAD | 60 |
| pSU41 | Kmr Plac | 61 |
| pMMB206 | Cmr Ptac Plac UV5 | 62 |
| pSW7848 | Exchange, oriVR6Kχ oriTRP4 araC-PBAD-ccdB | 63 |
| pHFAB | pomA and pomB in pBAD33 | 64 |
| pTSK155_2 | ΔflgBC (51–124) in pSW7848 | This study |
| pTSK157_2 | ΔflgCC (56–130) in pSW7848 | This study |
| pTSK160_2 | ΔflgFC (118–242) in pSW7848 | This study |
| pTSK161_2 | ΔflgGN (8–140) in pSW7848 | This study |
a
Rifr, rifampin resistant; Kmr, kanamycin resistant; Cmr, chloramphenicol resistant; Emr, erythromycin resistant; Smr, streptomycin resistant; Tpr, trimethoprim resistant; Tcr, tetracycline resistant; Pof+, normal polar flagellar formation; multi-Pof, multiple polar flagellar and/or basal body formation (hyperpolar flagella); Laf−, lateral flagellar formation defect; Mot−, motility defect; Fla−, flagellar formation defect; Plac, lac promoter; Ptac, tac promoter; PBAD, arabinose promoter.