Table 1. m6A modulator families.

Family	Abbreviation	Name	Function	Forward primer	Reverse primer
The “writers” of m6A modification	METTL3	Methyltransferase-like 3	METTL14, METTL3 form m6A methyltransferase complex. WTAP guided and localized the METTL3–METTL14 complex into nuclear speckles to efficiently methylate target RNAs	TTGTCTCCAACCTTCCGTAGT	CCAGATCAGAGAGGTGGTGTAG
	METTL14	Methyltransferase-like 14		GAACACAGAGCTTAAATCCCCA	TGTCAGCTAAACCTACATCCCTG
The “readers” of m6A modification	YTHDF1	YT521-B homology F1	YTHDF proteins directly bind and recognize m6A methylation on mRNAs, spatiotemporal control over RNA metabolism in a collaborative way; YTHDF1 can stimulate the translation of target mRNA, while YTHDF2 promotes mRNA decay; YTHDF3 regulates RNA metabolism	ACCTGTCCAGCTATTACCCG	TGGTGAGGTATGGAATCGGAG
	YTHDF2	YT521-B homology F2		AGCCCCACTTCCTACCAGATG	TGAGAACTGTTATTTCCCCATGC
	YTHDF3	YT521B homology F3		GCTATCCACCTAGTTCTCTTGGG	ATGCCAGGCACCTTACTCAAA
The “erasers” of m6A modification	FTO	Fat mass and obesity-associated protein	FTO is the first enzyme identified to demethylate RNA m3U and m6A	AACACCAGGCTCTTTACGGTC	TGTCCGTTGTAGGATGAACCC
	ALKBH5	AlkB homolog 5	ALKBH5 might serve as another demethylase and regulate m6A in mRNAs	CGGCGAAGGCTACACTTACG	CCACCAGCTTTTGGATCACCA

m6A, N6-methyladenosine; mRNA, messenger RNA; m3U, 3-methyluridine.