Figure 5.

FOXO1 inhibition by AS1842856 pre‐treatment prevented I/R‐induced mitophagy and improves mitochondrial biogenesis. (a) DRP1, OPA1, MFN1, and MFN2 protein levels in renal cortex in different groups were monitored by Western blot analysis. Immunoblots of representative samples were shown. Data are shown as mean ± SEM, n = 6 per group. ^* P < .05 versus sham, ^# P < .05 versus I/R. (b) Quantitative analysis of intracellular DRP1, OPA1, MFN1 and MFN2 proteins in HK2s treated with or without different concentrations of AS1842856 was analysed by Western blot analysis. Data are shown as mean ± SEM. n = 5 per group, ^* P < .05 versus control. (c) Quantitative analysis of PGC‐1α, TFAM, PARK2, and https://www.guidetopharmacology.org/GRAC/ObjectDisplayForward?objectId=2161 proteins in renal cortex were analysed by Western blot analysis. Data are shown as mean ± SEM. n = 6 per group, ^* P < .05 versus sham, ^# P < .05 versus I/R. (d) Quantitative analysis of intracellular PGC‐1α, TFAM, PARK2 and https://www.guidetopharmacology.org/GRAC/ObjectDisplayForward?objectId=2161 proteins in HK2s. Data are shown as mean ± SEM. n = 5 per group, ^* P < .05 versus control, ^# P < .05 versus H/R. (e) The protein levels of FOXO1, PGC‐1α, PARK2, https://www.guidetopharmacology.org/GRAC/ObjectDisplayForward?objectId=2161, DRP1 and MFN2 in HK2s transfected with Ad‐FOXO1 were monitored by Western blot analysis. Data are shown as mean ± SEM. n = 5 per group, ^* P < .05 versus control. (f) Morphology of mitochondria in HK2s was detected by transmission electron microscopy, n = 5 per group. (g) Relative mitochondrial DNA abundance in renal cortex of different groups were analysed by qPCR. Data sre shoen as mean ± SEM. n = 5 per group, ^* P < .05 versus sham, ^# P < .05 versus I/R