Table 3.
Maximal responses and EC50 values of agonist‐induced vasodilation in the absence or presence of chloroquine (CQ) in mouse coronary arteries dissected from Wt or TH mice
| Wt | TH | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| n | Vehicle | n | CQ | n | Vehicle | n | CQ | ||
| Max. response | |||||||||
| ACh | Untreated | 13 | 101.6 ± 2.8 | 9 | 84.4 ± 5.1* | 12 | 66.1 ± 8.1 | 8 | 85.8 ± 9.8 |
| L‐NAME + Indo | 7 | 55.3 ± 14.3 | 7 | 45.3 ± 16.1 | 7 | 27.5 ± 9.9 | 7 | 53.7 ± 5.0* | |
| TRAM34 + Apa + Indo | 6 | 93.0 ± 9.6 | 6 | 51.1 ± 17.0* | 5 | 35.4 ± 19.0 | 5 | 19.7 ± 13.2 | |
| SNP | Untreated | 5 | 107.1 ± 7.0 | 5 | 119.5 ± 9.1 | ||||
| ‐log (EC50) | |||||||||
| ACh | Untreated | 13 | 7.5 ± 0.3 | 9 | 7.0 ± 0.3 | 12 | 6.6 ± 0.3 | 8 | 7.1 ± 0.5 |
| L‐NAME + Indo | 7 | 7.4 ± 0.6 | 7 | 7.6 ± 0.4 | 7 | 8.2 ± 0.8 | 7 | 6.4 ± 0.5* | |
| TRAM34 + Apa + Indo | 6 | 7.6 ± 0.7 | 6 | 6.0 ± 0.5 | 5 | 6.0 ± 0.4 | 5 | 8.7 ± 0.1* | |
| SNP | Untreated | 5 | 7.7 ± 0.5 | 5 | 6.7 ± 0.4 | ||||
| n | Vehicle | n | 18GA | n | Vehicle | n | 18GA | ||
| Max. response | |||||||||
| ACh | L‐NAME + Indo + CQ | 8 | 37.1 ± 8.5 | 8 | 18.2 ± 3.6 | 8 | 41.9 ± 9.6 | 8 | 23.1 ± 3.2 |
| ‐log (EC50) | |||||||||
| ACh | L‐NAME + Indo + CQ | 8 | 5.4 ± 0.4 | 8 | 8.1 ± 0.8* | 8 | 6.1 ± 0.6 | 8 | 8.4 ± 0.8* |
Note. L‐NAME, 100 μM; Indo, 10 μM; TRAM34, 100 nM; Apa, 100 nM; vehicle for CQ, saline; 18GA, 50 μM; vehicle for 18GA, 0.25% ethanol. Data are mean ± SE.
*
P < .05 versus vehicle.