Table 4. Evaluation of oxidative damage markers in plasma using one-way analysis of dispersion (ANOVA).
Sampling time | Group | GSH [mmol/L] | NP-SH [mmol/L] | NP-SS-NP [mmol/L] | G-SS-NP [mmol/L] | MDA [mol/L] | 3-NT [µmol/L] |
---|---|---|---|---|---|---|---|
Before intubation | V (n=6) | 0.050±0.006* | 0.146 (0.132; 0.145) | 0.228±0.019 | 0.019±0.004 | 4.431 (4.382; 4.598) | 0.572±0.114* |
P (n=6) | 0.042±0.003* | 0.140 (0.139; 0.141) | 0.249±0.013 | 0.023±0.002 | 4.528 (4.398; 4.718) | 0.946±0.040* | |
Before clamp | V (n=6) | 0.050±0.004* | 0.145 (0.141; 0.153) | 0.227±0.019 | 0.020±0.004 | 4.492 (4.364; 4.630) | 0.659±0.140* |
P (n=6) | 0.041±0.003* | 0.138 (0.137; 0.140) | 0.257±0.011 | 0.024±0.003 | 4.729 (4.497; 4.992) | 0.959±0.061* | |
After ischemia | V (n=6) | 0.047±0.004 | 0.138 (0.134; 0.141) | 0.238±0.012 | 0.019±0.003 | 4.422 (3.734; 4.837) | 0.626±0.115* |
P (n=6) | 0.042±0.003 | 0.135 (0.134; 0.142) | 0.254±0.011 | 0.024±0.002 | 4.923 (4.681; 5.252) | 0.926±0.079* | |
After reperfusion | V (n=6) | 0.049±0.004* | 0.136 (0.132; 0.145) | 0.245±0.025 | 0.021±0.003 | 4.263 (3.597; 4.591)* | 0.528±0.047* |
P (n=6) | 0.041±0.004* | 0.142 (0.138; 0.144) | 0.243±0.014 | 0.025±0.002 | 4.951 (4.819; 5.141)* | 0.953±0.075* |
Values are expressed as medians (quartile Q0.25; quartile Q0.75) or as average ± standard deviation, statistically significant differences between groups at a significance level of P<0.050 are marked with *. V, intervention group; P, control group; GSH, glutathione; NP-SH, non-protein thiols; NP-SS-NP, non-protein disulfides; G-SS-NP, glutathione-mixed disulfides; MDA, malondialdehyde; 3-NT, 3-nitrotyrosine.