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. 2019 Dec;7(23):731. doi: 10.21037/atm.2019.11.126

Figure 2.

Figure 2

GENT ameliorated the inflammatory cytokine production of BMMs stimulated by LPS/IFN-γ. (A,B,C,D,E,F,G,H) BMMs were pretreated with GENT (500 or 1,000 µg/mL) or the same volume of PBS for 3 h, and then LPS (1 µg/mL)/IFN-γ (100 ng/mL) was added; 6 hours later, the RNA and supernatants of BMMs were isolated. (A,B,C,D,E,F) The mRNA levels of IL-1β, IL-6, TNF-α, iNOS, CCL5 and CXCL10 of BMMs were determined by qPCR. Error bars represent the mean ± SEM, representative of at least three experiments. (G,H) The protein levels of IL-6 and TNF-α in the supernatant of BMM were analyzed by ELISA assay. (I,J,K) RAW264.7 cells were pretreated with GENT (40 or 1000 µg/mL) or the same volume of PBS for 3 h, and then LPS (1 µg/mL)/IFN-γ (100 ng/mL) was added; 6 hours later, the RNA and supernatants of RAW264.7 were isolated. Data are represented as the mean ± SEM for three individual experiments. * indicates P<0.05. GENT, gentiopicroside; BMM, bone marrow-derived macrophage; LPS, lipopolysaccharide; IFN, interferon; PBS, phosphate buffer solution; IL, interleukin; TNF, tumor necrosis factor; ELISA, enzyme linked immunosorbent assay.