Figure 3.

GENT ameliorated the phosphorylation of IKKα/β and P65 without any obvious effect on MAPK signaling under LPS/IFN-γ stimulation. BMMs were pre-stimulated with GENT or DMSO for 3 hours, then treated with LPS (1 µg/mL)/IFN-γ (100 ng/mL). The phosphorylation of IKKα/β, P65, JNK, ERK, and P38 and the protein levels of IκBα and GAPDH were determined by Western blotting at 15, 30 and 60 minutes after LPS/IFN-γ stimulation. The experiment was repeated 3 times. The grouping of blots is due to cropping from the same batch of samples, although in different parts of different gels. GENT, gentiopicroside; LPS, lipopolysaccharide; IFN, interferon; BMM, bone marrow-derived macrophage.