Table 1. Sequences of primers used in the real-time polymerase chain reaction.
| Genes | Sequences of primers | GenBank accession number | Annealing Tm (nn) | Product size (bp) |
|---|---|---|---|---|
| IL-1β | F: 5' CTGGTACATCAGCACCTCAC 3', R: 5' AGAAACAGTCCAGCCCATAC 3' |
NM_008361.4 | 55 | 124 |
| IL-6 | F: 5' TGTATGAACAACGATGATGCACTT 3', R: 5' ACTCTGGCTTTGTCTTTCTTGTTATCT3' |
NM_001314054.1 | 60 | 197 |
| TNF-α | F: 5' AGTGACAAGCCTGTAGCCC 3', R: 5' GAGGTTGACTTTCTCCTGGTAT 3' |
NM_013693.3 | 57 | 252 |
| CXCL10 | F: 5' CCAAGTGCTGCCGTCATTTTC3', R: 5' GGCTCGCAGGGATGATTTCAA3' |
NM_021274 | 62.4 | 157 |
| CCL5 | F: TTTGCCTACCTCTCCCTCG3', R: CGACTGCAAGATTGGAGCACT3' |
NM_013653 | 61 | 85 |
| NOS2 | F: 5' GGAGTGACGGCAAACATGACT 3', R: 5' TCGATGCACAACTGGGTGAAC 3' |
NM_001313922.1 | 52 | 127 |
| p65 | F: 5' GATTGAAGAGAAGCGCAAAA 3', R: 5' CAGAAGTTGAGTTTCGGGTA 3' |
NM_009045.4 | 55 | 131 |
| β-actin | F: 5' CGTTGACATCCGTAAAGACC 3', R: 5' TAGGAGCCAGAGCAGTAATC 3' |
NM_007393.5 | 56 | 110 |
IL-1β, interleukin-1β; IL-6, interleukin-6; TNF-α, mouse tumor necrosis factor α; NOS2, inducible nitric oxide synthase 2; CXCL10, Mus musculus chemokine (C-X-C motif) ligand 10 (Cxcl10); CCL5, chemokine (C-C motif) ligand 5; F, forward primer; R, reverse primer.