Fig. 9.

(a) Schematic illustration of TSPNs prepared by nanoprecipitation. (b) Absorption spectra of TSPN in water (solid green line) and TII-TEG in dichloromethane (dashed purple line). The dip in the green curve is due to the strong absorption of water between 1400 and 1500 nm. In vivo PA/US imaging of TSPN in rat skin (c) without injection of TSPNs (upper) and with injection of $50\mu \mathrm{L}$, $40\mu \mathrm{g}/\mathrm{mL}$ matrigel inclusions of TSPNs (lower), and in mouse tumor (dashed circles); (d) without injection of TSPNs (upper) and with injection of $50\mu \mathrm{L}$, $40\mu \mathrm{g}/\mathrm{mL}$ aqueous solution of TSPNs (lower). The laser energy density is $\sim 10\mathrm{mJ}/{\mathrm{cm}}^2$ at 800 nm, $19\mathrm{mJ}/{\mathrm{cm}}^2$ at 1000 nm, $33\mathrm{mJ}/{\mathrm{cm}}^2$ at 1100 nm, $37\mathrm{mJ}/{\mathrm{cm}}^2$ at 1200 nm, and $25\mathrm{mJ}/{\mathrm{cm}}^2$ at 1300 nm. Each image is normalized with the corresponding laser energy density. Reproduced with permission from Ref. 169.