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. Author manuscript; available in PMC: 2020 Dec 6.
Published in final edited form as: J Mol Biol. 2019 Oct 15;431(24):4848–4867. doi: 10.1016/j.jmb.2019.10.005

Fig. 2.

Fig. 2.

Transitioning to recombinant NP C-terminal fragment binding studies. (a) Coomassie-stained SDS-PAGE of the nluc-NP612 fusion proteins with Western blotting using the sdAb-AP fusions. (b) Titrations of the nluc-NP612 fusion proteins over each of the oriented sdAb as captors. The legend is shown in the sdAb ZC graph and is the same for all panels. ELISA was performed on two different occasions, and error bars represent ±SD. (c) Determining EC50 values for the interaction of nluc-NP612 fusions with neutravidin-immobilized sdAb. (d) Determining EC50 values for the interaction of sdAb–gluc fusions with passively immobilized full-length NP polymers. ELISAs for (c) and (d) were performed on three different occasions, and error bars represent ±SD, with the EC50 values shown in the legend boxes. ELISA, enzyme-linked immunosorbent assay; m, molecular weight marker with sizes in kDa; NP, nucleoprotein; SD, standard deviation; BDBV, Bundibugyo ebolavirus; EBOV, Zaire ebolavirus; RESTV, Reston ebolavirus; SUDV, Sudan ebolavirus; TAFV, Taï Forest ebolavirus;sdAb, single-domain antibody; nluc, nanoluciferase.