Skip to main content
. Author manuscript; available in PMC: 2020 Dec 6.
Published in final edited form as: J Mol Biol. 2019 Oct 15;431(24):4848–4867. doi: 10.1016/j.jmb.2019.10.005

Fig. 3.

Fig. 3.

(a) Preparative SEC chromatograms of the final sdAb and sdAb + NP C-terminal complexes used for crystallization trials. (b) Coomassie Blue–stained SDS-PAGE analysis of 20 μg of the final concentrated protein preparations (except sdAb ZC, which was 10 μg). Molecular weight markers (kDa) are indicated on the left. (c)–(f) Overlays of various small-scale analytical SEC of various sdAb, NP634, and sdb + NP634 combinations relative to molecular weight markers; hen egg lysozyme (HEL, 14.3 kDa); carbonic anhydrase (CAH, 29 kDa); ovalbumin (OVA, 42.7 kDa); bovine serum albumin (BSA, 66.5 kDa). (c) sdAb ZC, EBOV NP634, and sdAb ZC + EBOV NP634. (d) sdAb ZE, EBOV NP634, and sdAb ZE + EBOV NP634. (e) sdAb ZE, SUDV NP634, and sdAb ZE + SUDV NP634. (f) sdAb SB, SUDV NP634, and sdAb SB + SUDV NP634. SEC, size-exclusion chromatography; EBOV, Zaire ebolavirus; SUDV, Sudan ebolavirus; sdAb, single-domain antibody.