Skip to main content
. Author manuscript; available in PMC: 2020 Dec 6.
Published in final edited form as: J Mol Biol. 2019 Oct 15;431(24):4848–4867. doi: 10.1016/j.jmb.2019.10.005

Fig. 8.

Fig. 8.

(a) View of the NP C-termini from the sdAb SB + SUDV NP610 complex with the “rightmost” domain (chain D) displayed with the V-shelf uppermost while the “leftmost” domain (chain B) appears almost 180° flipped in a head-over-tail fashion along the axes of the alpha helices of the shelf. Interface residues are colored as yellow sticks. The interface with sdAb (chain A) is shown for chain B (b) and chain D (c) with the partnering NP domain and other sdAb removed for clarity. sdAb interface residues are colored as follows: CDR1, orange; CDR2, green; CDR3, magenta; FR2, red; and other FR, salmon. (d) Electrostatic surfaces of two NP domains with one sdAb (the other removed for clarity) oriented in a “top-down” view aligned as in (a) with sdAb cartoon colored green, while one NP is cyan and the other is yellow. Rotating the assembly 90° and then 180° highlights the arrangement of one NP domain relative tothe other and the long CDR3 loop lodged in the intervening space of the latter. sdAb, single-domain antibody; SUDV, Sudan ebolavirus; NP, nucleoprotein; FR2, framework 2.