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. 2020 Jan 13;10:3115. doi: 10.3389/fmicb.2019.03115

FIGURE 3.

FIGURE 3

Gene-edited cell lines 87 and 4 maintain normal cell vitality and still fully resist PRRSV infection after cell passage. (A) Real-time monitoring of cell growth and vitality through the xCELLigence system (ACEA Biosciences, Inc., San Diego, CA, United States). A total of 10,000 cells of WT, 87, and 4 were seeded in an electronic microtiter plate and the cell index were continuously monitored by the xCELLigence system for 72 h. Statistical analysis was performed using an unpaired t-test (∗∗∗P < 0.001; ∗∗∗∗P < 0.0001). (B–D) Gene-edited cell lines 87 and 4 confer resistance to PRRSV after increasing the cell passages. Cells from WT, 87, and 4 at 4th (B), 6th (C), and 8th (D) cell passages were inoculated with PRRSV-EGFP (MOI = 1) for 48 h. Cells were then observed by fluorescence microscope (Bar: 200 μm).