FIGURE 5.
Colocalization between CHR6 virions and different marker identifying a specific compartment in the endocytic pathway or CD163 receptor of WT and CD163ΔSRCR5 cells. Cells were inoculated with CHR6 strain (MOI = 1) at 4°C for 1 h and subsequently incubated for different time points at 37°C. Cells were then fixed and permeabilized with 0.5% Triton X-100 for immunofluorescent staining with a mouse anti-PRRSV N antibody (green), a rabbit anti-CD163 antibody (red), and antibodies to different compartments of the endocytic pathway (red). Early endosome antigen 1 (EEA1) was stained to label early endosomes, the cation-independent mannose-6-phosphate receptor (CI-M6PR) was stained to label late endosomes, and lysosome-associated membrane protein 1 (Lamp1) was stained to label lysosomes. Representative co-localization (yellow) images are shown. Scale bar represents 5 μm.