Figure 2.

Whole-cell patch-clamp voltage clamp recordings under nine different protocols, which were all measured in each cell, at five temperatures. Each panel, from top to bottom, shows the voltage clamp protocol (black) and normalized current recordings (blue) that passed quality control at 25, 27, 30, 33, and 37°C, respectively. Currents were normalized with the method described in the text (see Fig. S1). (A) The calibration protocol and the staircase protocol are shown. (B–I) Shown are the eight different protocols used as validation of the model calibration, which are the activation current-voltage (I-V) protocol, the steady-state inactivation I-V protocol, the hERG screening protocol, the DAD-like protocol, the EAD-like protocol, and the cardiac action potential-like protocol at 0.5, 1, and 2 Hz, respectively. In (B and C), validation 1 and 2 show the I-V relations extracted from the currents. To see this figure in color, go online.