Figure 5.

Normalized whole-cell patch-clamp voltage-clamp recordings for 124 cells under nine different protocols and the model fitting and validation results. All currents are normalized by scaling them to minimize the absolute difference between each trace and a reference trace. From (A) to (I): the staircase protocol which is used as the calibration protocol, the activation I-V protocol, the steady-state inactivation I-V protocol, the hERG screening protocol, the DAD-like protocol, the EAD-like protocol, and the cardiac action-potential-like protocol at 0.5, 1, and 2 Hz, respectively, are shown. All the model calibration results and validation predictions are shown in the top panels (red) and are compared against the experimental recordings shown in the bottom panels (blue). Magnifications of the green shaded regions are shown underneath each panel to reveal the details of the spikes, in which our models show extraordinarily good predictions to the details. The normalized current for all protocols is shown except for the activation I-V protocol and the steady-state inactivation I-V protocol, in which the summary statistic I-V relationships are shown. To see this figure in color, go online.