Figure 4.

MPTP activity and Ca²⁺ cycling behavior in a single mitochondrion. The membrane potential is clamped at −80 mV. The free cytosolic Ca²⁺ maintains at ∼0.1 μM by adjusting the background Ca²⁺ current. The total simulated time is 300 s for each simulation. (A) Distributions of MPTP open duration and time when MPTP opens from experiments by Lu et al. (55) and our simulations are shown. (B) MPTP open frequency, open probability, and mean open duration from experiment by Lu et al. (55) and our simulations are shown. (C) Time course of mitochondrial free Ca²⁺ with MPTP opening and closing from experiment by Lu et al. (55) and our simulation in a single mitochondrion are shown. To see this figure in color, go online.