Figure 5.

Distinct Numerical, Phenotypical, and Transcriptional Characteristics of Mos from Nt2 Controller

(A) Left panel: box and whiskers plot showing the proportions of total CD14⁺ Mos among live PBMCs in Nt2 (orange, n = 21), Nt1 (yellow, n = 18), and NN (green, n = 16) controllers. The error bars represent minimum to maximum values. The lower panel shows the Spearman correlation between frequencies of total Mos and frequencies of total pTfh cells within CD4⁺ T cells in Nt controllers (Nt1, yellow; Nt2, orange). p and Spearman R values considering only Nt populations (blue) or all controllers including NNs (black) are shown on top of the plot.

(B) Spearman correlation between proportions of total Mos and potency of antibody neutralization of the indicated n = 6 tier 2 and tier 3 pseudoviruses in Nt2 (orange) and Nt1 (yellow) and NN (green) controllers. p and Spearman R values considering only Nt populations (blue) or all patients including NNs (black) are shown on top of the plot.

(C) Box and whiskers plots showing MFI of PD-L1+L2 (left), CD83 (center), and CD86 (right) in total Mos from Nt2 (orange, n = 21), Nt1 (yellow, n = 18), and NN controllers (green, n = 16). The error bars represent minimum to maximum values. ^∗∗p < 0.01 (Mann-Whitney test).

(D) Spearman correlations between the MFI of PDL1+L2 (left panels) CD86 (center panels), and CD83 (right panels) and plasma HIV-1 VL. p and Spearman R values considering only Nt populations (blue) or all patients including NNs (black) are shown on top of the plot.

(E and F) Spearman correlations between the MFI of PD-L1 (E) and CD86 (F) with the potency of neutralization of 6 HIV-1 pseudoviruses virus. FDR-corrected p and Spearman R coefficient for only Nts (blue) or all patients (black) are shown.