TABLE 3.

Comparisons of the quantities of amino acids taken up or released by S. aureus at the stationary phase of growth under optimal conditions (A) compared with growth under sub-optimal environmental conditions (B–D).

Amino	(A) Control pH7 at 37°C with no		(B) pH7 at 37°C with 2.5%		(C) pH6 and 35°C with no		(D) pH8 at 35°C with no
acids	added NaCl. nmole/100 μL		NaCl added. nmole/100 μL		added NaCl nmole/100 μL		added NaCl nmole/100 μL
	Taken up	Released	Taken up	Released	Taken up	Released	Taken up	Released
LEU	292 ± 1.2		272.9 ± 10.1*		273.9 ± 5.1*		338.4 ± 3.2*
LYS	154 ± 4.6		132.4 ± 17.9		48.5 ± 14.1*		112.6 ± 0.9*
SER	135 ± 1.8		116.4 ± 4.4*		141.7 ± 3.6*		147.9 ± 1.1*
ALA	108 ± 0.2		108.2 ± 0.0		109.4 ± 0.3		115.6 ± 0.1
GLU	98.8 ± 1.5		85.6 ± 0*		62.3 ± 1.4*		89.4 ± 0.7*
THR	86.9 ± 0.1		85.5 ± 0.7		108.8 ± 0.9*		100.7 ± 1.0*
PHE	63.8 ± 2.4		67.7 ± 9.2		71.5 ± 1.8		66.7 ± 0.5
ASN	52.0 ± 0.3		45.03 ± 0.2*		55.1 ± 0.7*		50.3 ± 0.5*
GLY	50.4 ± 0.0		51.5 ± 0.0*		55.5 ± 0.3*		53.2 ± 0.17*
MET	23.8 ± 1.4		24.4 ± 2.13		32.4 ± 0.7*		32.01 ± 0.9*
HIS	18.3 ± 0.9		29.0 ± 0.1*		26.01 ± 0.4*		22.8 ± 0.17*
PRO	14.6 ± 1.8		14.2 ± 0.8		15.8 ± 0.7*		12.8 ± 0.9*
GLN	12.7 ± 7.2		6.5 ± 10.5		19.01 ± 27.2		5.67 ± 22.8
ILE	11.8 ± 4.6		20.9 ± 2.5*		3.8 ± 2.4*		28.5 ± 1.79*
ASP	8.8 ± 1.2		11.7 ± 0.9*		29.7 ± 0.2*		28.4 ± 0.6*
TPR	6.6 ± 0.0		7.8 ± 0		9.4 ± 0.0		6.6 ± 0.0
AAA	6.3 ± 0.0		8.8 ± 0.0		4.3 ± 0.0		6.7 ± 0.0
TRP	5.5 ± 2.0		14.1 ± 4.2*		19.4 ± 1.2*		18.4 ± 1.6*
HYP	2.8 ± 0.9		2.3 ± 0.8		2.7 ± 0.6		2.5 ± 0.9
ABA	2.4 ± 0.3		4.2 ± 0.4*		5.1 ± 0.0*		3.6 ± 0.3*
ORN	1.3 ± 0.15			21.16 ± 1.5*		11.1 ± 0.9*	1.2 ± 0.1
VAL		81.8 ± 6.5		46.69 ± 7.2*		91.3 ± 5.6		76.5 ± 1.6
TYR		17.0 ± 3.7		6.04 ± 3.8*		4.8 ± 3.8*		13.7 ± 3.7

The amino acids have been ranked in the order of their magnitudes of uptake measured at optimal conditions (mean ± SD, P < 0.05). *Indicates amino acids were significantly uptaken or released.