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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: DNA Repair (Amst). 2019 Sep 21;83:102720. doi: 10.1016/j.dnarep.2019.102720

Figure 4. Overexpression of SpExo5 causes checkpoint activation and cell death.

Figure 4.

(A) Overexpression of spExo5 leads to cell death in S. pombe cells regardless of checkpoint proficiency. Cells either WT or rad3Δ containing pREP3x-spExo5 or pREP3x-spExo5-D207A were grown overnight in EMM-leu supplemented with 5μM thiamine. Cells were washed serially diluted on plates either containing 15 μM or 0 μM thiamine and incubated 2-3 day at 30 °C. (B) Light microscopy of cells from (A), either grown on plates containing 15 μM or 0 μM thiamine for 2-3 days.