Fig. 5.

MiR-517a inhibition enhances OS of cells via upregulation of CDKN1C and suppression of the JNK signaling pathway. a, b Western blot analysis of Cleaved Caspase-3, Bax/Bcl-2 ratio and extent of ERK2 phosphorylation in cells treated with 250 μmol/L H₂O₂. c EdU staining of cells treated with 250 μmol/L H₂O₂ (×200). d Proliferation rate of cells treated with 250 μmol/L H₂O₂. e Membrane potential changes of cells treated with 250 μmol/L H₂O₂ under a fluorescence microscope (×400). f, g Apoptosis rate of cells treated with 250 μmol/L H₂O₂. h Levels of T-AOC, ROS, and MDA along with SOD activity in cells treated with 250 μmol/L H₂O₂; *p < 0.05 compared with cells without treatment; ^#p < 0.05 compared with cells treated with NC inhibitor. All data were expressed as mean ± standard deviation; comparisons among multiple groups were analyzed by one-way ANOVA; the experiment was repeated three times